Vol. 6, Issue 2, Part A (2024)

The spread of drug-resistant pathogens is one of the most serious threats to successfully treating microbial diseases. Evaluating plant extracts and plant-derived products for antimicrobial potential has demonstrated that plants could serve as a promising source of new antibiotic prototypes. The current research was conducted to assess the antimicrobial efficacy of Phellodendron amurense stem bark extract on clinical isolates of Escherichia coli, Pseudomonas aeruginosa, Candida albicans, Staphylococcus aureus, Klebsiella pneumoniae, and Bacillus sp. Using a two-fold serial dilution, concentrations of 100, 50, 25, 12.5, and 6.25 mg/mL of the solution were generated and used for the antimicrobial assay. The antimicrobial properties of the crude extract of Phellodendron amurense stem bark were assessed by employing the agar well diffusion principles. From the result, the extracts effectively inhibited the growth of S. aureus, E. coli, and P. aeruginosa, but did not have any effect on the remaining organisms. At 100 mg/ml concentration, the extract of P. amurense exhibited the highest activity against P. aeruginosa, followed by E. coli and S. aureus, with an inhibitory zone diameter of 8.67 mm, 8 mm, and 5.67 mm, respectively with no activity observed on the other organisms at the various concentrations tested. Phytochemical analysis of the stem extract of Phellodendron amurense revealed the presence of alkaloids, tannins, saponins, and triterpenoids but no flavonoids. The result from this research has shown that different bacteria exhibited varying degrees of sensitivity and resistance to the extract of Phellodendron amurense.